Antibody Development
Comprehensive Solutions for Developable lead candidates
Antibody Discovery is only half the journey. Identifying and optimizing the perfect lead candidate requires thorough functional validation and robust developability assessment. To ensure long-term success, antibodies must not only bind their target but also demonstrate functional activity, stability, and manufacturability. These critical steps determine whether a promising molecule can become a true development candidate.
THREE Steps fast track from target to lead
- Effector function: ADCC, CDC
- ADC testing: cytotoxicity
- Binding studies: ELISA, BLI, flow cytometry
- Affinity measurement
- Epitope binning and mapping
- Other functional tests: receptor inhibition, internalization, competition assays, immune activation, checkpoint inhibition
- SEC-HPLC
- HIC-HPLC
- Melting temperature
- Antibody self-interaction (AC-SINS)
- Polyreactivity
- Stability: serum, buffer, pH & temperature
